I once had a job applicant who spent 10 minutes responding to this question. She told me how awful her boss was and how her company was a terrible place to work. It so happened that her boss was a good friend and golfing buddy of my boss - our company's CEO - and the company was one of our biggest clients. Of course, she didn't get the job.
Don't make the same mistake she did. Instead, accentuate the positive and minimize any difficult situations. Discuss the strengths your past supervisors had and how they helped you succeed in your positions.
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